Fig. 3
From: CRISPR/Cas9 targeting of GPRC6A suppresses prostate cancer tumorigenesis in a human xenograft model
Editing GPRC6A attenuated osteocalcin-induced intracellular signaling activation in PC-3 cells. a Comparison of Arg, osteocalcin and testosterone activated ERK phosphorylation in PC-3 control, PC-3-sgRNA1, PC-3-sgRNA3 cells. Dose dependent effect of osteocalcin on ERK phosphorylation (b), AKT phosphorylation (c), and mTOR phosphorylation (d) was blocked in PC-3 cells with CRISPR/Cas9 editing of the GPRC6A gene locus. Protein intensity in the Western blot was analyzed by Image J. * and ** represent significant differences from control group and stimulated groups at p < 0.05 and p < 0.01 (n ≥ 4)