Table 1 Primers for qRT-PCR
From: CRISPR/Cas9 targeting of GPRC6A suppresses prostate cancer tumorigenesis in a human xenograft model
Transcript | Primer sequences | Product length (bp) | Annealing temp(°C) | Accession Number |
|---|---|---|---|---|
GPRC6A | F:CCGGGACATATCATAATTGGG R:CATTGCCACTGTGACTTCTGT | 234 | 58 | NM_148963 |
PCNA | F:CCTGCTGGGATATTAGCTCCA R:CAGCGGTAGGTGTCGAAGC | 109 | 61 | NM_002592 |
c-Fos | F:GAGGGAGCTGACTGATACAC R:CTCGGGGTAGGTGAAGACGA | 568 | 60 | NM_005252 |
MMP2 | F:TACAGGATCATTGGCTACACACC R:GGTCACATCGCTCCAGACT | 90 | 60 | NM_004530 |
MMP9 | F:TGTACCGCTATGGTTACACTCG R:GGCAGGGACAGTTGCTTCT | 97 | 58 | NM_004994 |
VEGF | F:CAAGACAAGAAAATCCCTGTGG R:CCTCGGCTTGTCACATCTG | 161 | 58 | M32977 |
BMP3 | F:GCAGGGAGAGAGACCGAAG R:TGGACCGTGCTGTACCTGT | 163 | 58 | NM_001201 |
PSA | F:GTGTGTGGACCTCCATGTTATT R:CCACTCACCTTTCCCCTCAAG | 160 | 60 | AJ512346 |
RUNX2 | F:TCAACGATCTGAGATTTGTGGG R:GGGGAGGATTTGTGAAGACGG | 81 | 58 | NM_001015051 |
OCN | F:GGCGCTACCTGTATCAATGG R:GTGGTCAGCCAACTCGTCA | 110 | 60 | NM_199173 |
HSD17B11 | F:CTGGCGAAGTGCGTGAGATT R:GAGTACGCTTTCCCAATTCCAT | 133 | 58 | NM_016245 |
HSD3B1 | F:CACATGGCCCGCTCCATAC R:GTGCCGCCGTTTTTCAGATTC | 90 | 58 | NM_000862 |
AKR1C3 | F:TCTGGGATCTCAACGAGACAA R:TGGAACTCAAAAACCTGCACG | 207 | 58 | NM_003739 |
Cyclophilin A | F:CTGCACTGCCAAGACTGAGTG R:CCACAATGTTCATGCCTTCT | 77 | 58 | NM_008907 |