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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Collagen triple helix repeat containing-1 negatively regulated by microRNA-30c promotes cell proliferation and metastasis and indicates poor prognosis in breast cancer

Fig. 4

CTHRC1 is a direct target of miR-30c. a qRT-PCR analysis of CTHRC1 mRNA expression in indicated cells 24 h post-transfection. **P < 0.01. b CTHRC1 protein expression was detected by western blot in indicated cells post-transfection. c Wild type (Wt) and Mutant type (Mut) CTHRC1 3’UTR sequences were cloned into a psi-CHECK2 reporter vector. d The relative luciferase activity was detected by dual-luciferase reporter assay in indicated cells. **P < 0.01

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