Fig. 2

Hypermethylation of NF2 in GBM and malignant glioma cell lines. The methylation status of NF2 was detected in normal brain and in GBM (n = 5). N, normal brain; G, GBM (a). The methylation status of NF2 was detected in HEB, U251, U87, T98-G and A172 cell lines (b). Increased NF2 promoter methylation in GBM brain samples compared with normal brain samples was detected by bisulfite Sanger sequencing (c); the exact methylation sites are indicated (d)