Fig. 4

DNMT1 negatively regulates the expression of NF2. Levels of mRNA expression of DNMT1, NF2, and miR-152-3p after transfection with DNMT1 siRNA and pcDNA-DNMT1 detected by RT-PCR in U251 cells (a). Protein expression of DNMT1 and NF2wasdetected by Western blot (b) and immunofluorescence (c). After DNMT1 knockdown, the methylation status of NF2 in U251 cells was determined with MSP (d) and bisulfite Sanger sequencing (e). *, P < 0.05, **, P < 0.01 compared with the control groups treated with negative control (NC) siRNA. ##, P < 0.01 compared with control groups treated with pcDNA