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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: miR-500a-3p promotes cancer stem cells properties via STAT3 pathway in human hepatocellular carcinoma

Fig. 2

miR-500a-3p targets multiple negative regulators of STAT3 signaling. a Predicted miR-500a-3p target sequence in 3′UTRs of SOCS2, SOCS4, SOCS6, PTPN4 and PTPN11 b Western blotting of SOCS2, SOCS4, SOCS6, PTPN4 and PTPN11 expression after transfected with miR-500a-3p mimics or miR-500a-3p inhibitor compared to control . α-Tubulin served as the loading control. c and d Real-time PCR analysis of SOCS2, SOCS4, SOCS6, PTPN4 and PTPN11 expression in Hep G2 and Huh 7 cells transduced with miR-500a-3p mimics or transfected with miR-500a-3p inhibitor compared to control. Transcript levels were normalized by GAPDH expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. e and f Luciferase assay of cells transfected with pmirGLO-3′UTR reporter in the indicated Hep G2 and Huh 7 cells, respectively. g and h MiRNP IP assay showing the association between miR-500a-3p and SOCS2, SOCS4 and PTPN11 transcripts in Hep G2 and Huh 7 cells. Pulldown of IgG antibody served as the negative control

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