Fig. 7

Clinical relevance of miR-500a-3p with SOCS2, SOCS4 and PTPN11 in hepatocellular carcinoma tissues. a Real-time PCR of miR-500a-3p expression in eight fresh hepatocellular carcinoma tissues. U6 was used as the control for RNA loading; miRNA levels were normalized to that miR-500a-3p expression of sample one. Each bar represents the mean ± SD of three independent experiments. b Western blotting of SOCS2, SOCS4 and PTPN11 expression in the same eight hepatocellular carcinoma tissues. α-tubulin was used as the loading control. c-e Correlation between miR-500a-3p levels and SOCS2, SOCS4 and PTPN11 expression in hepatocellular carcinoma clinical tissues. The expression levels of SOCS2, SOCS4 and PTPN11 were quantified by densitometry using Quantity One Software, and normalized to the levels of α-tubulin. The sample one was used as a standard. The relative expressions of these proteins were used to perform the correlation analysis