Fig. 1

CDK16 was down-regulated in HCC. a Quantitative RT-PCR analysis of CDK16 expression in 72 pairs HCC tissues and their corresponding adjacent livers and 14 normal livers (left panel). Up-regulation of CDK16 (>2 fold) was detected in 46% (33/72) of primary HCCs, including 9 cases showed a high up-regulation (>4 fold) (right panel). b The relative mRNA level of CDK16 in HCC cell lines (HepG2, Hep3B, HepG2, Huh7, PLC/PRF/5, SK-hep1, HCCLM3 and HCCLM9) and human normal liver cell (L02). c Representative IHC images showing the protein level of CDK16 in HCC (top) and paired adjacent non-tumor tissue (bottom). d CDK16 mRNA expression was elevated in a stepwise manner along disease progression. e Kaplan-Meier analysis of OS and RFS based on CDK16 expression levels in 72 patients with HCC. f CDK16 expression in 50 pairs of HCC samples (up) and Kaplan-Meier analysis of OS in 365 HCC patients (down) from TCGA dataset. *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments