Fig. 3

CDK16 knockdown represses proliferation, induces cell cycle arrest and apoptosis. a CDK16 knockdown efficiency was evaluated by RT-qPCR (top) and western blotting (bottom) in Huh7 and HCCLM9 cell lines. b and (c) CCK 8 and colony formation assay showing that silencing of CDK16 inhibited the proliferation of HCC cell lines. d FACS analysis showing cell cycle arrest in G2/M phase after silencing of CDK16 in Huh7 and HCCLM9 cell lines. e The apoptosis of Huh7 and HCCLM9 cells increased after CDK16 knockdown. f Representative images of tumors removed from nude mice implanted with pLenti-siCDK16 transfected HCCLM9 cells (n = 5) or control cells (n = 5) (left panel) and tumors stained with Ki67 (right panel). *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments