Fig. 4

CDK16 induces epithelial-mesenchymal transition (EMT) in hepatoma cells. a Knockdown of CDK16 in Huh7 and HCCLM9 cells suppressed cell invasion as revealed by transwell invasion assay. b Wound-healing assay showing reduced migration ability after CDK16 knockdown. c CDK16 overexpression induced EMT in L02 cells. d Expression levels of EMT markers (E-cadherin, claudin-1, N-cadherin, vimentin, and fibronectin) and MMPs (MMP-2,MMP-9) in HCCLM9 and Huh7 cells after CDK16 knockdown were analyzed by western blotting. e Knockdown of CDK16 changed the expression of EMT markers and MMP-2 and MMP-9 in tumor xenografts of nude mice. *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments