Fig. 5

CDK16 regulates HCC cell invasion through the GSK3β/β-catenin pathway. a Knockdown of CDK16 reduced expression of β-catenin via regulation of GSK3β in vitro and in vivo. b CDK16 overexpression enhances the level of β-catenin through inactivation of GSK3β. c Immunofluorescence images show that overexpression of CDK16 promotes translocation of β-catenin in Huh7 and HCCLM9 cells. d Western blotting assay show that nuclear β-catenin was decreased after CDK16 knockdown. e Expression of EMT markers in CDK16 overexpression cells with or without β-catenin knockdown. f Transwell assays performed using Huh7 and HCCLM9 cells after cotransfection with vector or CDK16 and control or β-catenin siRNA. *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments