Fig. 6

Hyperactivation of E2F1 facilitates CDK16 up-regulation in human HCC. a Venn plot shows 8 transcriptional factors with putative binding sites in promotor region of CDK16 from Jaspar and PROMO database (left panel), and their correlations with CDK16 were analyzed using pearson correlation analysis (right panel). b E2F1 is frequently up-regulated in human HCC (left panel) and is positively correlated with CDK16 expression in TCGA HCC sample set (right panel). c CDK16 is up-regulated after E2F1 knockin in mice model (GSE71380). d Knockdown of E2F1 reduced expression of CDK16 at mRNA (left panel) and protein level (right panel). e Left panel: Schematic illustration of E2F1 binding site on CDK16 promotor and the mutant CDK16 promotor. Right panel: Inactivation of E2F1 by siRNA significantly reduced wild type but not mutant CDK16 promoter activity in Huh7 cells. f Chromatin immunoprecipitation (ChIP) assays used normal IgG or anti-E2F1 to identify E2F1 binding sites on the CDK16 promoter in Huh7 cells. *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments