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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: RETRACTED ARTICLE: Up-regulation of CDK16 by multiple mechanisms in hepatocellular carcinoma promotes tumor progression

Fig. 7

miR-125b-5p negatively regulates CDK16 expression in human HCC. a MicroRNA target prediction programs identified miR-125b-5p as a post-transcriptional regulator of CDK16. b miR-125b-5p was significantly down-regulated in HCC in TCGA LIHC datasets (left panel) and was negatively correlated with CDK16 expression (right panel). c Immunostaining of CDK16 protein in HCC samples show negative correlation between CDK16 and miR-125b-5p. miR-125b-5p expression is measured by qRT-PCR. d Overexpression of miR-125b-5p inhibited endogenous CDK16 mRNA and protein expression level in Huh7 and HCCLM9 cells. e CDK16 mRNA and protein levels after inhibition of miR-125b-5p. f CDK16 is a direct target of miR-125b-5p. Schematic diagram illustrates miR-125b-5p binding site within CDK16 3’UTR and its mutated version (left), luciferase reporter assay shows that overexpression of miR-125b-5p suppressed reporter signal of wild-type CDK 3’UTR but not mutant (right). *p < 0.05; **p < 0.01; ***p < 0.001. All results are from at least three independent experiments

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