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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis

Fig. 1

H ferritin gene silencing in MCF-7 cells. a Real-time PCR analysis of FHC mRNA expression was performed on total RNA extracted from MCF-7shRNA and MCF-7shFHC cells. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Student t-test (****, p < 0.0001). Western Blot analysis for FHC expression was performed on 50 μg of total protein extracts from MCF-7shRNA and MCF-7shFHC cells. γ-Tubulin was used as loading control. Representative data from one of three experiments. b MCF-7shRNA and MCF-7shFHC cells were fixed and incubated with monoclonal anti-FHC antibody (1:200) followed by incubation with the appropriate secondary antibody. Nuclei were visualized by DAPI staining. Images were collected using a Leica TCS SP2 confocal microscopy system (63X). Representative data from one of three experiments. c Images, collected using the phase-contrast microscope (Leica DMI 6000 CS), were used to highlight morphological changes between MCF-7shRNA and MCF-7shFHC cells. Representative data from one of three experiments. d MCF-7shRNA and MCF-7shFHC cells were fixed and incubated with Oregon Green 488 phalloidin (1:400) to visualize actin filaments. Nuclei were visualized by DAPI staining. Images were collected using confocal microscopy system (40X). Representative data from one of three experiments

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