Fig. 3
From: Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis
EMT markers and FHC amounts in MCF-7 cells. a Real-time PCR analysis of FHC was performed on total RNA extracted from MCF-7shRNA/pcDNA 3, MCF-7shFHC/pcDNA 3 and MCF-7shFHC/pcDNA 3 FHC cells. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Student t-test (**, p < 0.01, ****, p < 0.0001). Western Blot analysis of FHC was performed on 50 μg of total protein extract from MCF-7shRNA/pcDNA 3, MCF-7shFHC/pcDNA 3 and MCF-7shFHC/pcDNA 3 FHC cells. γ-Tubulin was used as loading control. Representative data from one of three experiments. b Real-time PCR analysis of E-Cadherin, Vimentin, Twist and Slug were performed on total RNA extracted from MCF-7shRNA/pcDNA 3, MCF-7shFHC/pcDNA 3 and MCF-7shFHC/pcDNA 3 FHC cells. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Student t-test (*, p < 0.05, **, p < 0.01, ***, p < 0.001, n.s., not significant)