Fig. 4
From: Epithelial-to-mesenchymal transition in FHC-silenced cells: the role of CXCR4/CXCL12 axis
MCF-7shFHC cells shows a more aggressive phenotype. a Wound healing assay was conducted to measure migration capacity of MCF-7shRNA and MCF-7shFHC cells. Images of cellular migration were taken at times 0 h, 24 h and 48 h (magnification of 10X) using the Leica DFC420 C and Leica Application Suite Software. Wound opening was quantified by ImageJ 64 software. Final results represent mean ± SD of three independent experiments. Cell migration fold change was evaluated using the T0 of MCF-7shRNA as control. Statistical significance was evaluated by Two-Way ANOVA (Sidak’s) (n.s. not significant, **, p< 0.01). b Cell viability was assessed in MCF-7shRNA and MCF-7shFHC cells using the MTT method, as indicated in the Methods section, at 24 h, 48 h and 72 h. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Two-Way ANOVA (Sidak’s) (n.s. not significant, *, p < 0.01, ***, p < 0.001). c Cells were seeded in 10 ml of DMEM in 100 mm plates. After 24 h, 48 h and 96 h, 500 μl of supernatant were taken and glucose and lactate concentration was measured. Final results represent mean ± SD of three independent experiments. Statistical significance was evaluated by Two-Way ANOVA (Sidak’s) (**, p < 0.01, ***, p < 0.001, ****, p < 0.0001)