Fig. 1

Wogonoside suppresses breast cancer growth and metastasis of MDA-MB-231 cells in vivo. a Diagram shows the experimental course of MDA-MB-231 orthotopic model. b Effect of wogonoside (80 mg/kg) and gemcitabine (80 mg/kg) on tumor growth was investigated in the model of MDA-MB-231 orthotopic model. c Effect of wogonoside (80 mg/kg) and gemcitabine (80 mg/kg) on tumor weight was investigated in the model of MDA-MB-231 orthotopic model. d H&E stained brains, lungs, livers, bones and spleens of mice from wogonoside-treated and control group to confirm the presence of micrometastases (image magnification: 200×). e Immunohistochemical detection of E-cadherin, MMP-9, vimentin and Twist1 protein levels in MDA-MB-231 orthotopic site (image magnification: 400×). f The expression of E-cadherin, MMP-9, vimentin and Twist1 proteins were analyzed in MDA-MB-231 orthotopic site by western blot using specific antibodies. A GAPDH antibody was used to check equivalent protein loading. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group