Fig. 2

TNF-α and TRAF2/4 were overexpressed in late stage of metastatic breast cancer. a Diagram shows the experimental course of MDA-MB-231 orthotopic model (n = 8). b The effect of wogonoside on TNF-α content of tumor tissue. c Immunohistochemical detection of TNF-α protein levels in 63-day and 98-day MDA-MB-231 orthotopic site (image magnification: 400×). d The expression of TRAF4, TRAF2 and TNF-α proteins were analyzed in 63-day and 98-day MDA-MB-231 orthotopic site by western blot using specific antibodies. A GAPDH antibody was used to check equivalent protein loading. e Immunohistochemical detection of TRAF4, TRAF2 and TNF-α protein levels in MDA-MB-231 orthotopic site (image magnification: 400×). f The expression of TRAF4, TRAF2 and TNF-α proteins were analyzed in MDA-MB-231 orthotopic site by western blot using specific antibodies. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group