Fig. 5

Wogonoside inhibits TNF-α-induced NF-κB signaling through suppressing the expression of TRAF2/4. MDA-MB-231 and MDA-MB-435 cells were exposed to different concentrations of TNF-α and wogonoside for 48 h. a The expression of p-IKKα, IKKα, p-IκBα and IκBα proteins in the cells were analyzed by western blot using specific antibodies. b The expression and localization of NF-κB p65 protein in the cells was analyzed by western blot using specific antibodies. c The expression of TRAF2 protein in the cells was analyzed by western blot using specific antibodies. d The expression and localization of TRAF4 protein in the cells was analyzed by western blot using specific antibodies. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group