Fig. 7

Wogonoside inhibits Twist1 expression through suppressing TRAF2/4 expression in MCF7 cells. MCF7 cells were exposed to different concentrations of TNF-α, TGF-β1 and wogonoside for 48 h. a TRAF4, TRAF2 and Twist1 expression in MDA-MB-231, MCF7, MDA-MB-435, and BT-474 cells were analyzed by western blot using specific antibodies. Comparison was made with the expression in MDA-MB-231 cells. b MCF7 cells were transfected with TRAF4, TRAF2 and Twist plasmid. TRAF4, TRAF2 and Twist1 expression with treatment of wogonoside were analyzed by western blot. c The expression of TRAF2 and Twist1 protein in the cells were analyzed by western blot using specific antibodies. d The expression and localization of TRAF4 protein in the cells was analyzed by western blot using specific antibodies. e The expression of p-IKKα, IKKα, p-IκBα and IκBα proteins in the cells were analyzed by western blot using specific antibodies. f The expression and localization of NF-κB p65 protein in the cells was analyzed by western blot using specific antibodies. Each experiment was performed at least three times. Data are presented as mean ± SD. *p < 0.05 compared with the control group; **p < 0.01 compared with the control group