Fig. 6

Mutant p53 promoted the expression of stathmin. After wtp53, mutp53 (R175H, G245C and R282W) plasmids and vectors were transfected into SCC25 cells, cells transfected with mutp53 grew more aggressively than those with wtp53 (a). In SCC25 cells transfected with pcDNA3.1 vector, pcDNA3.1-wtp53 and pcDNA3.1-mutp53 for 48 h, upregulation of stathmin was found in cells transfected with mutp53 but not wtp53 (b). Decreased stathmin expression was found accompanied by p53 knockdown for 48 h in two mutant p53 cell lines, HN6 (H179L) and HN13 (V173 L) (c). Decreased stathmin mRNA levels were found accompanied by knockdown of p53 for 48 h in HN6 and HN13 cells but not wtp53 HN30 cells (d, e). **: P < 0.01, *: P < 0.05