Fig. 4From: Maintenance of stemness is associated with the interation of LRP6 and heparin-binding protein CCN2 autocrined by hepatocellular carcinomaExpression of LRP6 in HCC is related to malignant phenotypes. a LRP6 was silenced in MHCC97H cells using specific vshRNA, and LRP6-Sh2 was validated as yielding the most efficient interference of LRP6 by western blot. Expression of CCN2 and β-catenin was determined following the downregulation of LRP6 in MHCC97H cells and following gene rescue of LRP6. b Invasiveness, migration, colony formation, and the anoikis ability were assessed among MHCC97H–Mock, MHCC97H-shRNA-LRP6, and cells and LRP6 rescued cells. c Subcutaneous tumor growth capacity of MHCC97H-shRNA-LRP6 cells and of MHCC97H–Mock cells in nude mouse model was assessedBack to article page