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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Maintenance of stemness is associated with the interation of LRP6 and heparin-binding protein CCN2 autocrined by hepatocellular carcinoma

Fig. 5

CCN2 activated Wnt signaling and enhanced malignant potential through the upregulation and phosphorylation of LRP6. Analysis of expression of LRP6 and p-LRP6, phosphorylation level of β-catenin, and expression of the stemness-related markers SOX2 and CD90 according to (a) increasing concentrations (0–2000 ng/ml) and (b) time (0–48 h) of CCN2. c Invasiveness, migration, and adherent colony formation were assessed in CCN2-treated HCC cell lines MHCC97H and Hep3B. d Expression of GSK3β, P-GSK3β (Ser9), β-catenin, and SOX2 was assessed in CCN2 (250 ng/ml)-treated MHCC97H cells following treatment with the specific LRP6 inhibitor DKK1 (100 ng/ml) for 24 h. e Wnt signaling associated proteins GSK3β, P-GSK3β (Ser9), β-catenin, and SOX2 were assessed in the LRP6-knockdown cell line MHCC97H–LRP6-Sh2, which was treated with CCN2 in increasing concentrations (0, 250, 1000 ng/ml)

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