Fig. 2

Effect of shRNA on Girdin gene expression in hepatoma cells. a, b Fluorescence microscopy (×100, upper panel, indicating successful transfection), Protein expression (middle panel), and mRNA expression (lower panel) of Girdin in HepG2 (a) and Huh-7 cells (b). Mean ± SD (n = 3 independent experiments). ** P < 0.01 and *** P < 0.001. NC, normal control; Girdin-shRNA, shRNA transfected cells