Fig. 7

Girdin knockdown inhibits the expression of glycolysis-related proteins and the PI3K-Akt signaling pathway. a, b Immunoblots showing the levels of Glut1, LDH-A, HK2, HIF-1α, AKT, p-AKT, and PI3K in HepG2 (a) and Huh-7 cells (b), with or without Girdin-shRNA treatment, but without radiation. Mean ± SD (n = 3 independent experiments). *, P < 0.05 and **, P < 0.01. NC, normal control; Girdin-shRNA, shRNA transfected cells; Glut1, Glucose transporter 1; LDH-A, Lactic dehydrogenase-A; HK2, Hexokinase 2; HIF-1α, Hypoxia inducible factor 1α; AKT. Protein-serine-threonine kinases; p-AKT, phosphorylated AKT; PI3K, phosphatidylinositol 3-kinase