Fig. 1

Global identification of a multiple drug resistance associated proteome. a. Experimental strategy for global identification of a multiple drug resistance associated proteome. SK-N-BE(1) and SK-N-BE(2) were labeled with K0R0 and K4R6, respectively. Equal number of cells were mixed and lysed. Extracted proteins were digested into tryptic peptides, which were pre-separated and analyzed by HPLC/MS/MS analysis. Proteins were subsequently identified and quantified. b. Parson correlation analysis of the quantified proteins between the two replicates. c. Volcano plot summarizing protein quantification results in terms of protein changes (log2 fold change > 1 indicates upregulation in the resistant cell line more than two-fold) and significance (p value of 0.05 is indicated by the red line)