Fig. 4

ANXA2 knockdown induced more apoptosis with chemotherapeutic agents and activated the apoptotic pathway. a, b. After treated with doxorubicin (0.5 μM) or etoposide (10 μM) for 48 h, Hoechest 33342 staining showed significantly increased numbers of apoptotic cells in ANXA2 knockdown group compared with the control group. c, d. AnnexinV/PI flow cytometric analysis showed significantly increased apoptosis in the ANXA2 knockdown group compared with control group following etoposide treatment. Each quantitative bar chart represents the sum of quadrant 2 and 4 (n = 3 independent experiments). e~g. After treatment with doxorubicin (0.5 μM) and etoposide (10 μM) for 48 h, pro-apoptotic proteins AIF and Cleaved-PARP were increased, particularly in ANXA2 knockdown groups. The plotted error bars represent mean ± SEM from 3 independent experiments. *: p < 0.05, **: p < 0.01