Fig. 5

ANXA2 could regulate the nuclear translocation of p50 following treatment with TNF-α. a~d. The change of subunits (p50, p65) of NF-κB family in SK-N-BE(2) cells treated without or with TNF-α (100 ng/ml) for 1 h. Cytosol and nuclear fractions were prepared special extraction assay. Densitometric analysis showed subunit p50 was involved in NF-κB activation. e~g. Under the same stimulation of TNF-α, densitometric analysis clearly showed the subunit p50 was downregulated in the nucleus of ANXA2 knockdown NB cells compared with control cells following treatment with TNF-α *: p < 0.05, **: p < 0.01, ***: p < 0.001, The plotted error bars represent mean ± SEM from 3 independent experiments