Fig. 6

ANXA2 could regulate the activation of NF-κB through combined with the subunit p50. a, b. The change in localization of endogenous p50 was analyzed by IF-IC following treatment with or without TNF-α (100 ng/ml) for 1 h. Nuclei were stained with DAPI. The nuclear localization of endogenous p50 in each group were quantitated as a graph. The plotted error bars represent mean ± SEM. The nuclear localization of p50 was quantified according to data from 5 random figures in each group. c. Interaction between ANXA2 and p50 in SK-N-BE(2) cells was validated through Co-IP and analyzed by western blotting with the indicated antibodies **: P < 0.01. All results are from at least three independent experiments