Fig. 2

miR-493 increases the sensitivity of lung cancer cells to cisplatin in vitro. a miR-493 overexpression increases the sensitivity of A549 and A549/DDP cells to cisplatin. The IC50 of miR-493 overexpressed lung cancer cells was significantly lower than that of the control (P < 0.01). b miR-493 overexpression enhances cisplatin-induced apoptosis. A549 and A549/DDP cells were treated with 5 μg/mL cisplatin for 24 h. Cell apoptotic death events were monitored by Annexin V/PI staining and flow cytometry assays. Each experiment was independently repeated at least 3 times. Error bars correspond to the mean ± SD. (*p < 0.01). c miR-493 enhances DDP-induced DNA damage in lung cancer cells. Immunofluorescence staining was performed to examine γ-H2AX foci formation. A549 and A549/DDP cells were treated with 5 μg/mL cisplatin for 24 h. γ-H2AX foci formation was detected by fluorescence microscope. γ-H2AX foci were counted in 5 random 200× micro fields and categorized into the indicated groups. Representative images of the assays are shown. Original magnification: ×200.Significant differences are indicated, (*, P < 0.05)