Fig. 3

Combination of salirasib and Exo2 suppress migration, invasion and apoptosis of prostate cancer cells more efficiently than either drug alone. a DU145 and PC3 cells were treated with 50 μM Exo2 and 50 μM salirasib for 16 h, alone or in combination, and cell migration was determined by gap closure. b–d DU145 and PC3 cells were treated with 50 μM Exo2 and 50 μM salirasib for 24 h, alone or in combination. Cell invasion was determined by Boyden chamber (b), and cell apoptosis was determined by FITC Annexin V Apoptosis Detection Kit I (c). For quantification of invasion, the matrigel membranes that contained invading cells were dissolved in 10% acetic acid and read colorimetrically at 590 nm (b). Representative images of apoptosis assays are shown in (c) and quantitative data are shown in (d). e DU145 and PC3 cells were treated with 50 μM Exo2 and 50 μM salirasib for 24 h, alone or in combination, and cell lysates were collected for Western blot with the indicated antibodies. **p < 0.01