Skip to main content
Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: (S)-crizotinib induces apoptosis in human non-small cell lung cancer cells by activating ROS independent of MTH1

Fig. 2

(S)-crizotinib induces ROS in NSCLC cells independent of MTH1. a-c Intracellular ROS generation as assessed by DCF fluorescence in cells following exposure to (S)-crizotinib for different time periods (a, 30 μM) and different concentrations (b). Quantification of data in (b) is shown in panel (c) [*P < 0.05 compared to DMSO control]. d-f Levels of nitric oxide production in NSCLC cells as measured by DAF-FM fluorescence. Cells were treated as outlined in a-c. Quantification of data in (e) is shown in panel (f) [*P < 0.05, **P < 0.01 compared to DMSO control]. g-h Effect of MTH1 knockdown on ROS production (DCF fluorescence) in NCI-H460 cells [**P < 0.01, ns = no significance]. i-l NCI-H460 cells were treated with 30 μM (S)-crizotinib with or without 1 h pretreatment with 5 mM NAC (i) or 5 mM GSH (k) The relative mean fluorescence intensity is presented in (j) and (l) [*P < 0.05 and **P < 0.01]

Back to article page