Fig. 3

Blocking ROS abolishes the cytotoxicity of (S)-crizotinib. a-c NSCLC cells were pre-treated with 5 mM NAC (a-b) or 5 mM GSH (c) for 1 h before exposure to 30 μM (S)-crizotinib for 24 h. Extent of cell apoptosis was determined by annexin-V/PI staining. b and c show quantification of flow cytometry data [*P < 0.05 and **P < 0.01]. d Apoptosis-related proteins were measured in NCI-H460 and H1975 cells following treatment as outlined in (a) and (b). GAPDH was used as internal control