Fig. 4
From: Combination therapy of PKCζ and COX-2 inhibitors synergistically suppress melanoma metastasis
The combination of J-4 and Celecoxib severely affected melanoma cells adhesion and F-actin formation. (a and b) Adhesion assay results in B16-F10 (a) and A375 (b) cells at 5, 15 and 30 min after various treatments for 24 h. Cell numbers in five fields were counted for each coverlip under the microscopy with 200 × magnitudes. (c and d) 20 ng/ml of EGF induced F-actin formation in B16-F10 (c) and A375 (d) cells were severely inhibited by the combination of J-4 and Celecoxib. (e) Confocal images of B16-F10 and A375 cells after various treatments. F-actin was stained with rhodamine labeled phalloidin. EGF: 20 ng/ml; J-4: 25 μM; Celecoxib: 25 μM. * P < 0.05; ** P < 0.01