Table 1 Primer Sequences and Reaction Properties
From: Combination therapy of PKCζ and COX-2 inhibitors synergistically suppress melanoma metastasis
Target | Sequence | Product Size (bp) | Annealing temp. (°C) | |
|---|---|---|---|---|
Human | ||||
PKCζ | forward | CTGAGGAGCACGCCAGGTT | 625 | 58.1 |
reverse | ACGGGCTCGCTGGTGAACT | |||
COX-2 | forward | TCTGCAGAGTTGGAAGCA-CTCTA | 216 | 58.4 |
reverse | GCCGAGGCTTTTCTACCAGAA | |||
β-actin | forward | CTGGCACCCAGCACAATG | 458 | 54.1 |
reverse | GCCGATCCACACGGAGTACT | |||
Mouse | ||||
PKCζ | forward | ACGGACAACCCTGACATGAAC | 361 | 57.1 |
reverse | ATTCGGACTGGTCGATCCTCT | |||
COX-2 | forward | TCAGGTCATTGGTGGAGAGG | 96 | 54 |
reverse | GCAAACTGCAGGTTCTCAGG | |||
β-actin | forward | ATGGAGCCACCGATCCACA | 426 | 56.2 |
reverse | CATCCGTAAAGACCTCTATGCCAAC |