Fig. 1

Cancer patient sera-treated cells differentiated into the same lineages of the primary cancers. BRCA1-KO fibroblasts were treated for 3 weeks with sera from patients with CRC-LM (a Case272), HCC (b Case271), Pancreatic cancer (Pancreatic C.; c Case290116), or Ovarian cancer (Ovarian C.; d Case343). Treated cells were resuspended in HBSS/Matrigel mixture and injected subcutaneously into NOD/SCID mice (n = 3 mice per group). Four weeks after cells transplantation, mice were euthanized and the xenotransplants excised. Formalin-fixed paraffin-embedded tumors were processed for H&E staining, or immunolabeled with antibodies against tumor specific markers (Additional file 1: Table S1). Scale bar: 100 μm