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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Exosomes isolated from cancer patients’ sera transfer malignant traits and confer the same phenotype of primary tumors to oncosuppressor-mutated cells

Fig. 2

Cancer patient sera-isolated exosomes are efficiently internalized by target cells. a Representative micrographs of transmission electron microscopy on cancer patient sera exosome preparations. The image showed small vesicles of approximately 50–120 nm in diameter. Scale bars 100 nm. b Nanosight exosomes size analyses. Exosomes size was centered on 91 nm in diameter. Data are expressed as concentration average (black line) +/− standard error (red lines) of six measurements and are representative of 3 exosome preparations. c Proteins isolated from fibroblasts, or serum-isolated exosomes were analyzed by Western blot for the expression of specific markers. d Confocal microscopy monitoring of PKH-26-labeled exosome uptake in vitro into BRCA1-KO fibroblasts. Note that exosomes were dispersed in the cytoplasm and tended to form aggregates in the perinuclear regions. Scale bars 20 μm

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