Fig. 2

TFP interrupts autophagy flux of GBM cells. a Western blot showing expression of LC3BI/II in U251 and U87 cells incubated for 24 h with TFP at increasing concentrations. b Images of LC3 puncta in GBM cells transfected with pSELECT-GFP-LC3 and treated with PBS and 10 μM TFP for 48 h. c Images from transmission electron microscopy showing characteristic autophagic vacuoles (Black arrows) in GBM cells after treatment with PBS or 10 μM TFP for 48 h. d and e Western blot showing p62 levels in GBM cells after treatment with increasing concentrations of TFP, 100 nM bafilomycin A1, 2.5 μM rapamycin and 10 μM TFP for 48 h. **P < 0.01; ***P < 0.001; size bars in b = 10 μm, size bars in c = 1 μm