Fig. 3

TFP influences the acidification of lysosomes and increases the radiosensitivity of GBM cells. a LysoTracker Red in cells after treatment with PBS, 100 nM bafilomycin A1 and 10 μM TFP for 48 h. Nuclei are stained with Hoechst. b and c Comet assays to evaluate DNA damage in cells after treatment with PBS, 5 μM TFP, or 4 Gy radiation for 48, or pretreated with 5 μM TFP for 24 h combined with 4 Gy radiation and TFP for another 24 h. d Western blot for γ-H2AX in GBM cells after treatment. **P < 0.01; size bars = 50 μm