Fig. 5

TFP decreases HR repair and causes synergistic anticancer effect with radiation in GBM cells. a Western blots for Rad51, BRCA1, BRCA2 and γ-H2AX in U251 and U87 cells after different doses of TFP treatment. b HR activity of U251 and U87 after treatment of PBS and 5 μM TFP. c Colony formation of U251 and U87 after treatment with PBS, 5 μM TFP, or 4 Gy radiation for 48 h, or pretreated with 5 μM TFP for 24 h combined with 4 Gy radiation and TFP for another 24 h. d Caspase 3/7 activation of GBM cells in four treatment groups. **P < 0.01; ***P < 0.001; size bars = 50 μm