Fig. 5

ERK and JNK activation mediated Sophoridine-induced S phase arrest and apoptosis, respectively. a After 20 μM Sophoridine treatment, the protein levels of total or phosphorylated MAPK members (JNK, p38, ERK) were detected by western blotting. b Cancer cells were pretreated with 20 mM MEK inhibitor (PD98059) and JNK inhibitor (SP600125) before the treatment with or without 20 μM Sophoridine for 48 h. The protein levels of total or phosphorylated ERK and JNK were detected by western blotting. c, d After pre-treated with the indicated inhibitor for 1 h, cancer cells were treated with 20 μM Sophoridine for 48 h. The cell cycle and cell apoptosis analysis was performed. e, f The indicated protein levels in treated cancer cells were assessed by western blotting