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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Sophoridine induces apoptosis and S phase arrest via ROS-dependent JNK and ERK activation in human pancreatic cancer cells

Fig. 6

Sophoridine promotes the generation of ROS, which contributes to S phase cell cycle arrest and apoptosis in cancer cells. a Sophoridine in time-dependent manner upregulated ROS levels in Miapaca-2. After pretreated with 5 mM NAC for 2 h, cells were treated with or without 20 μM Sophoridine for indicated time, and then the intracellular levels of ROS were measured. b, c The treated cancer cells were sent to cell cycle and cell apoptosis analysis by FACS. d-f After pretreated with 5 mM NAC for 2 h, cells were treated with or without 20 μM Sophoridine for indicated time. The proteins of Cyclin A, Cyclin D1, Bcl-2, Bax, cleaved caspase-3, PARP, p-ERK and p-JNK in control or treated cancer cells were assessed by western blotting. **, P < 0.01 versus the NAC-untreated group

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