Fig. 7
Sophoridine inhibits pancreatic cancer cell growth in vivo. a 2 × 106 Miapaca-2 cells were inoculated into Balb/c nude mice. Mice were randomized into three groups (n = 7) and treated with PBS, or Sophoridine at the dose of 20 mg/kg or 40 mg/kg daily for 3 weeks. Tumor volumes were measured by digital caliper every 3 days. b The tumors were excised from mice after last treatment. c Tumor masses were weighed and compared, and each histogram represented the mean ± S.D. d The body weight was measured every week. e The phosphorylation signals of p-ERK, p-JNK and PCNA in xenograft tumor tissues were detected by immunohistochemistry. Apoptosis cells in mice tumor were detected by Tunel staining (f) The liver and kidney biochemical functions were evaluated. The AST, ALT and Cr levels of mouse blood were detected by Elisa assay. g The liver and kidney from control and Sophoridine group were stained with H&E to evaluate the toxicity after treatment. The histological structure of liver and kidney were observed and compared microscopically. h Overview of signaling pathways for Sophoridine-induced cell cycle arrest and apoptosis in human pancreatic tumors