Establishment of the targeted NGS platform to detect DNA alteration using DNA samples and cancer cell lines. The reference standard DNA samples and the genomic DNA from cancer cells were sequenced by the targeted NGS. The distribution of detected SNV by mutation allele frequencies (MAFs) was illustrated in (a) and (b). The scatter plots in (c) and (d) represent the consistancy between measured MAFs and expected MAFs. The sensitivities of SNV detection were shown in (e) and (f). Error bars, s.e.m.