Fig. 1

Annexin A2 enhances cisplatin resistance of lung cancer cells. a Annexin A2 expression of A549/DDP, A549, H460 and H1650 cells was analyzed by Western blot. β-Actin was employed as an inner control. b A549/DDP cells were transfected with Annexin A2 siRNA, Annexin A2 expression was analyzed by Western blot. c A549/DDP cells transfected with Annexin A2 siRNA were treated with cisplatin at the indicated concentration for 48 h, cell viability was measured by MTT assay. d A549/DDP cells transfected with Annexin A2 siRNA were treated with cisplatin at the indicated concentration for 14 days, (Left) Colonies were fixed with acetic acid-methanol (1:4) and stained with crystal violet. (Right) The number of colonies was from three independent experiments. e A549 cells were transfected with pCMV6-Annexin A2, Annexin A2 expression was analyzed by Western blot. f A549 cells transfected with pCMV6-Annexin A2 were treated with cisplatin at the indicated concentration for 48 h, cell viability was measured by MTT assay. Table indicates the IC50 values for each condition. g A549 cells transfected with pCMV6-Annexin A2 were treated with cisplatin at the indicated concentration for 14 days, (Left) Colonies were fixed with acetic acid-methanol (1:4) and stained with crystal violet. (Right) The number of colonies was from three independent experiments. *P < 0.05