Fig. 6

Annexin A2 inhibited p53 expression through c-Jun-mediated suppression of p53 promoter activity. a A549/DDP cells were transfected with Annexin A2 siRNA, c-Jun protein expression was analyzed by Western blot. b A549 cells were transfected with pCMV6-Annexin A2, and then treated with SP600125, c-Jun protein expression was analyzed by Western blot. c A549/DDP cells were transfected with Annexin A2 siRNA for 48 h, the binding capacity of c-Jun to p53 promoter were determined by chromatin immunoprecipitation. The enrichment of the p53 promoter in immunoprecipitated (IP) c-Jun and the input of c-Jun are shown. IgG was used as a negative control. One representative data set of three individual experiments is shown. d-e HEK293T cells (d) and A549 cells (e) were co-transfected with pGL3-p53-Luc, or mut-pGL3-p53 and pCMV-Annexin A2 for 48 h, cells were harvested and assayed by the Dual-Luciferase Reporter Assay System. *P < 0.05