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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway

Fig. 4

In vivo promotion of the aggressiveness of astrocytomas by overexpression of NUSAP1. a Representative images (left panel) and quantification (right panel) of the indicated invaded cells by the anchorage-independent growth assay. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). b Survival curves of mice with brain astrocytoma xenografts formed by the indicated cells (*P < 0.05). c Representative images of the tumor formed from the indicated cells in the intracranial brain tumor xenograft nude mice. Immunohistochemistry staining (upper panel) with the NUSAP1 antibody and hematoxylin-eosin staining (lower panel) demonstrated that overexpression of NUSAP1 induced, whereas downregulation of NUSAP1 inhibited, the aggressive phenotype of astrocytoma cells in vivo. d Western blotting was used to assess the expression of NUSAP1, MMP2, MMP9 and Ki67 in the indicated cells. GAPDH was used as a loading control

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