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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathway

Fig. 5

Activation of Hedgehog signaling by overexpression of NUSAP1. a Gene set enrichment analysis (GSEA) showed that there was a significant correlation between NUSAP1 mRNA expression and the Hedgehog pathway gene signature. b Western blotting was used to examine the expression of NUSAP1 and GLI1 in the indicated cells. β-actin and EF1-α were used as loading controls. c Relative activity of reporter luciferase linked to NUSAP1 and GLI1 in the indicated cells. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). d real-time-PCR detection of PTCH1, HIP1, CCND1, CCNE1 and HDAC1 gene expression in SW 1008 and U-87 MG cells. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05)

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