Fig. 6From: Nucleolar and spindle associated protein 1 promotes the aggressiveness of astrocytoma by activating the Hedgehog signaling pathwayPromotion of tumor aggressiveness by NUSAP1 overexpression via activation of Hedgehog signaling. a RT-PCR detection of GLI1 expression in the indicated cells. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). b Representative images (left panel) and quantification (right panel) of the indicated cells in the colony formation assay. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). c Representative images (left panel) and quantification (right panel) of the indicated invaded cells analyzed by the Transwell matrix penetration assay. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). d Representative images (left panel) and quantification (right panel) of the indicated invaded cells analyzed by the anchorage-independent growth assay. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05). e Luciferase reporter assays to determine GLI1 activity in indicated cells when treated with purmorphamine or not. Error bars represent the mean ± SD values of three independent experiments (*P < 0.05)Back to article page