Fig. 1

The acidic environment leads to increased expression of ASIC2. SW480 and SW620 cells were cultured in pH 6.5 medium for 24 h. a ASIC1, ASIC2, ASIC3, and ASIC4 mRNA expression in SW480 or SW620 cells was analyzed by qRT-PCR. Data are shown as the fold change of expression in the acidic medium compared with that in normal medium (mean ± SEM). b Immunofluorescence staining was used to determined ASIC2 expression in SW480 and SW620 cells after acidic treatment. c Western blotting was performed to determine ASIC2 expression in SW480 and SW620 cells after acidic treatment. Results are representative of three independent experiments. **p < 0.01 compared with control. Scale bar, 200 μM